p53 Antibody / TP53 [clone DO-1] (V8132)

Western blot testing of human HeLa cell lysate with p53 antibody (DO-1). Expected molecular weight ~53 kDa.

IHC staining of FFPE human colon carcinoma with p53 antibody (clone DO-1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using p53 antibody (clone DO-1). These results demonstrate the foremost specificity of the DO-1 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free p53 antibody (clone DO-1) as confirmation of integrity and purity.