p504S Antibody / AMACR [clone 13H4] (V8770)

Description

p504S antibody clone 13H4 is a monoclonal antibody that detects alpha-methylacyl-CoA racemase (AMACR), an enzyme localized in mitochondria and peroxisomes that participates in branched-chain fatty acid and bile acid metabolism. In clinical pathology, AMACR is better known as p504S, a highly reliable biomarker of prostate cancer. Its consistent upregulation in malignant prostate tissue, combined with limited or absent expression in benign glands, has made p504S one of the most widely used immunohistochemical markers for distinguishing malignant from benign lesions. NSJ Bioreagents provides p504S antibody clone 13H4 to support prostate disease research, oncology, and diagnostic pathology.

The antibody produces strong cytoplasmic staining in prostatic adenocarcinoma cells, making it a dependable marker in diagnostic panels. Pathologists frequently use it alongside basal cell markers such as p63 or high molecular weight cytokeratin antibodies. This combination improves diagnostic confidence by identifying the absence of basal cells and the presence of AMACR, features that together confirm malignant transformation.

In oncology research, the antibody has been applied to studies of prostate tumor progression, aggressiveness, and biochemical recurrence. Elevated AMACR expression correlates with advanced disease and has been linked to tumor cell metabolic reprogramming. By highlighting AMACR expression, this antibody provides insight into how cancer cells exploit altered lipid metabolism for survival and growth.

Although best known for its role in prostate pathology, p504S antibody clone 13H4 has broader applications. AMACR overexpression has been documented in colorectal carcinomas, renal cell carcinomas, and certain hepatocellular lesions. Researchers use the antibody to explore how metabolic dysregulation extends across cancer types, often highlighting AMACR as a potential biomarker beyond the prostate.

This antibody is also valuable in metabolic biology. AMACR catalyzes a key step in the β-oxidation of branched-chain fatty acids and bile acid intermediates. Altered activity has been linked to inborn metabolic errors, and detection with this antibody supports studies into rare disorders where AMACR deficiency plays a role.

Validated in tissue-based systems, the antibody consistently produces clear cytoplasmic staining with minimal background. Its reproducibility has made it a mainstay in prostate cancer diagnostics and a reliable tool in metabolic and oncologic research. Alternate names include AMACR antibody, alpha-methylacyl-CoA racemase antibody, prostate cancer marker p504S antibody, and mitochondrial/peroxisomal metabolism antibody.

Application Notes

Optimal dilution of the p504S antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Full length human recombinant protein was used as the immunogen for the p504S antibody.

Storage

Store the p504S antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).