P4HA1 Antibody / Prolyl 4-Hydroxylase Subunit Alpha 1 Antibody (R33435)

P4HA1 Antibody HEK293 WB. Western blot analysis was performed on HEK293 cell lysate using P4HA1 Antibody / Prolyl 4-Hydroxylase Subunit Alpha 1 Antibody at 0.2 ug/ml. A prominent immunoreactive band is detected at approximately 60 kDa, consistent with the expected molecular weight of P4HA1, together with an additional band at approximately 48 kDa. Both bands are abolished by pre-incubation of the antibody with the immunizing peptide, confirming their specificity. These results demonstrate that this P4HA1 Antibody / Prolyl 4-Hydroxylase Subunit Alpha 1 Antibody is suitable for specific Western blot detection of endogenous P4HA1 in HEK293 cell lysates.

P4HA1 Antibody Human Skin IHC. Immunohistochemistry was performed on FFPE human skin tissue using P4HA1 Antibody / Prolyl 4-Hydroxylase Subunit Alpha 1 Antibody at 5 ug/ml. Heat-induced epitope retrieval was carried out by steaming sections in pH 6 citrate buffer prior to staining. Binding was visualized using an alkaline phosphatase detection system with a red chromogen. Distinct cytoplasmic staining is observed predominantly within the basal and suprabasal layers of the epidermis, consistent with expression of P4HA1 in metabolically active keratinocytes involved in extracellular matrix maintenance and collagen biosynthesis. These results demonstrate that this P4HA1 Antibody / Prolyl 4-Hydroxylase Subunit Alpha 1 Antibody is suitable for immunohistochemical detection of endogenous P4HA1 in formalin-fixed, paraffin-embedded human skin tissue.

P4HA1 Antibody Human Spleen IHC. Immunohistochemistry was performed on FFPE human spleen tissue using P4HA1 Antibody at 5 ug/ml. Heat-induced epitope retrieval was carried out by steaming sections in pH 6 citrate buffer prior to staining. Binding was visualized using an alkaline phosphatase detection system with a red chromogen. Distinct cytoplasmic staining is observed in a subset of splenic cells, with strongest labeling in cells of the red pulp, consistent with endogenous expression of P4HA1 in metabolically active stromal and hematopoietic cell populations. These results demonstrate that this Prolyl 4-Hydroxylase Subunit Alpha 1 Antibody is suitable for immunohistochemical detection of endogenous P4HA1 in formalin-fixed, paraffin-embedded human spleen tissue.