P4HA1 Antibody / Collagen Biosynthesis Enzyme Antibody (FY13116)

P4HA1 Antibody Human Liver Cancer IF. Immunofluorescence was performed on FFPE human liver cancer tissue using P4HA1 Antibody (red). Heat-induced epitope retrieval was carried out in pH 8 EDTA buffer prior to incubation with the primary antibody at 5 ug/ml. A Cy3-conjugated goat anti-rabbit IgG secondary antibody was used for detection, and nuclei were counterstained with DAPI (blue). Strong cytoplasmic staining is observed throughout the tumor cells, consistent with the endoplasmic reticulum localization of P4HA1 as a key enzyme involved in collagen biosynthesis and extracellular matrix remodeling. These results demonstrate that this Collagen Biosynthesis Enzyme Antibody is suitable for immunofluorescent detection of P4HA1 in formalin-fixed, paraffin-embedded human liver cancer tissue.

Western blot analysis of P4HA1 using anti-P4HA1 antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P4HA1 antibody at 1:1000 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. A specific band was detected for P4HA1 at approximately 61 kDa. The expected molecular weight of P4HA1 is ~61 kDa.

P4HA1 Antibody Human Bladder Cancer IHC. Immunohistochemistry was performed on FFPE human invasive urothelial carcinoma of the bladder with squamous differentiation using P4HA1 Antibody. Heat-induced epitope retrieval was carried out in pH 8 EDTA buffer prior to incubation with the primary antibody at a 1:100 dilution. Bound antibody was detected using an HRP-conjugated goat anti-rabbit IgG secondary antibody with DAB chromogen. Moderate to strong cytoplasmic staining is observed within the neoplastic cells, consistent with elevated P4HA1 expression associated with collagen biosynthesis, extracellular matrix remodeling, and tumor progression. These results demonstrate that this Collagen Biosynthesis Enzyme Antibody is suitable for immunohistochemical detection of P4HA1 in formalin-fixed, paraffin-embedded human bladder carcinoma tissue.

P4HA1 Antibody Human Lung Cancer IHC. Immunohistochemistry was performed on FFPE human lung cancer tissue using P4HA1 Antibody. Heat-induced epitope retrieval was carried out in pH 8 EDTA buffer prior to incubation with the primary antibody at a 1:100 dilution. Bound antibody was detected using an HRP-conjugated goat anti-rabbit IgG secondary antibody with DAB chromogen. Moderate to strong cytoplasmic staining is observed throughout the neoplastic epithelial cells, consistent with increased P4HA1 expression associated with collagen biosynthesis, extracellular matrix remodeling, and tumor progression. These results demonstrate that this Collagen Biosynthesis Enzyme Antibody is suitable for immunohistochemical detection of P4HA1 in formalin-fixed, paraffin-embedded human lung cancer tissue.

Western blot analysis of P4HA1 using anti-P4HA1 antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human K562 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P4HA1 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. A specific band was detected for P4HA1 at approximately 61 kDa. The expected molecular weight of P4HA1 is at 61 kDa.

P4HA1 Antibody Human Liver Cancer IHC. Immunohistochemistry was performed on FFPE human liver cancer tissue using P4HA1 Antibody. Heat-induced epitope retrieval was carried out in pH 8 EDTA buffer prior to incubation with the primary antibody at 2 ug/ml. Bound antibody was detected using an HRP-conjugated goat anti-rabbit IgG secondary antibody with DAB chromogen. Moderate to strong cytoplasmic staining is observed throughout the malignant hepatocytes, consistent with elevated P4HA1 expression associated with collagen biosynthesis, extracellular matrix remodeling, and hypoxia-driven tumor progression. These results demonstrate that this Collagen Biosynthesis Enzyme Antibody is suitable for immunohistochemical detection of P4HA1 in formalin-fixed, paraffin-embedded human liver cancer tissue.

P4HA1 Antibody Human Liver IHC. Immunohistochemistry was performed on FFPE human liver tissue using P4HA1 Antibody. Heat-induced epitope retrieval was carried out in pH 8 EDTA buffer prior to incubation with the primary antibody at 2 ug/ml. Bound antibody was detected using an HRP-conjugated goat anti-rabbit IgG secondary antibody with DAB chromogen. Moderate diffuse cytoplasmic staining is observed in hepatocytes, consistent with the constitutive expression of P4HA1 as an essential enzyme involved in collagen biosynthesis and extracellular matrix homeostasis. These results demonstrate that this Collagen Biosynthesis Enzyme Antibody is suitable for immunohistochemical detection of P4HA1 in formalin-fixed, paraffin-embedded human liver tissue.

P4HA1 Antibody Human Ovarian Cancer IHC. Immunohistochemistry was performed on FFPE human ovarian cancer tissue using P4HA1 Antibody. Heat-induced epitope retrieval was carried out in pH 8 EDTA buffer prior to incubation with the primary antibody at a 1:100 dilution. Bound antibody was detected using an HRP-conjugated goat anti-rabbit IgG secondary antibody with DAB chromogen. Moderate to strong cytoplasmic staining is observed throughout the neoplastic cells, consistent with elevated P4HA1 expression associated with collagen biosynthesis, extracellular matrix remodeling, and tumor progression. These results demonstrate that this Collagen Biosynthesis Enzyme Antibody is suitable for immunohistochemical detection of P4HA1 in formalin-fixed, paraffin-embedded human ovarian cancer tissue.

P4HA1 Antibody U-2 OS Cell IF. Immunofluorescence was performed on U-2 OS cells using P4HA1 Antibody (red). Enzyme-mediated antigen retrieval was performed prior to incubation with the primary antibody at 5 ug/ml. A Cy3-conjugated goat anti-rabbit IgG secondary antibody was used for detection, and nuclei were counterstained with DAPI (blue). Strong cytoplasmic staining with a reticular, perinuclear distribution is observed, consistent with the endoplasmic reticulum localization of P4HA1 as a key enzyme involved in collagen biosynthesis and post-translational collagen modification. These results demonstrate that this Collagen Biosynthesis Enzyme Antibody is suitable for immunofluorescent detection of endogenous P4HA1 in human U-2 OS cells.