p40 Antibody / Epithelial Stratification and Differentiation Marker Antibody (V8182)

p40 Antibody. Immunohistochemistry analysis of FFPE human cervix tissue demonstrates strong HRP-DAB brown nuclear staining in basal and parabasal epithelial cells, with progressively reduced staining in more differentiated superficial layers. The nuclear-restricted pattern reflects epithelial layering and supports use of this antibody as an epithelial stratification and differentiation marker, highlighting the transition from proliferative basal compartments to differentiated cell populations. Staining is well-defined with minimal background, allowing clear visualization of epithelial organization and differentiation gradients. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to antibody incubation.

p40 Antibody. Immunohistochemistry analysis of FFPE human breast carcinoma tissue demonstrates focal HRP-DAB brown nuclear staining in tumor cell clusters, while surrounding stromal elements remain largely negative. The nuclear-restricted pattern highlights epithelial cells with basal-like characteristics and supports use of this antibody as an epithelial stratification and differentiation marker, reflecting deltaNp63-associated regulation of epithelial layering and cell state. Staining is heterogeneous across tumor regions, allowing identification of p40-positive subpopulations within the carcinoma. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to antibody incubation.