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Home >> Antibodies >> p38 gamma Antibody / MAPK12

p38 gamma Antibody / MAPK12 (RQ6495)

  Catalog No Formulation Size Price (USD)  
Image RQ6495 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human HeLa cells with p38 gamma antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human HEK293, 3) monkey COS-7, 4) human K562, 5) rat skeletal muscle and 6) mouse skeletal muscle lysate with p38 gamma antibody. Expected molecular weight: 38-42 kDa.
Flow cytometry testing of human U-2 OS cells with p38 gamma antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= p38 gamma antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P53778
Localization Cytoplasmic, nuclear
Applications Western blot : 1-2ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This p38 gamma antibody is available for research use only.
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Description

Mitogen-activated protein kinase 12 (MAP kinase 12), also known as p38 gamma, extracellular signal-regulated kinase 6 (ERK6) or stress-activated protein kinase 3 (SAPK3), is an enzyme that in humans is encoded by the MAPK12 gene. Activation of members of the mitogen-activated protein kinase family is a major mechanism for transduction of extracellular signals. Stress-activated protein kinases are one subclass of MAP kinases. The protein encoded by this gene functions as a signal transducer during differentiation of myoblasts to myotubes.

Application Notes

Optimal dilution of the p38 gamma antibody should be determined by the researcher.

Immunogen

An E. coli-derived human protein (amino acids S229-L367) was used as the immunogen for the p38 gamma antibody.

Storage

After reconstitution, the p38 gamma antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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