P2RY5 Antibody / Lysophosphatidic Acid Receptor Antibody (RQ4219)

P2RY5 Antibody Human Ovarian Teratoma IHC. Immunohistochemical analysis of FFPE human ovarian teratoma tissue using P2RY5 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody at 2 ug/mL overnight at 4°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Distinct membranous and cytoplasmic staining is observed in epithelial cells within the teratoma, consistent with LPAR6/P2RY5 expression. These results demonstrate that P2RY5 Antibody, a Lysophosphatidic Acid Receptor Antibody, specifically detects endogenous receptor expression in FFPE human tissue.

P2RY5 Antibody HepG2 Cell IF. Immunofluorescent staining of HepG2 cells using P2RY5 Antibody. Cells underwent enzyme-mediated antigen retrieval for 15 minutes before incubation with the primary antibody at 5 ug/mL overnight at 4°C. A Fluoro488-conjugated goat anti-rabbit secondary antibody was used for detection, and nuclei were counterstained with DAPI (blue). Green fluorescence is predominantly localized to the cell membrane and cytoplasm, consistent with the expected distribution of the membrane-associated LPAR6/P2RY5 receptor. These results demonstrate that P2RY5 Antibody, a Lysophosphatidic Acid Receptor Antibody, specifically detects endogenous receptor expression in cultured human cells.

P2RY5 Antibody Human Cell WB. Western blot analysis of human HaCaT, RT4, and T47D cell lysates using P2RY5 Antibody. Lane 1: HaCaT whole cell lysate. Lane 2: RT4 whole cell lysate. Lane 3: T47D whole cell lysate. Samples (30 ug/lane) were separated on a 10% SDS-PAGE gel under reducing conditions, transferred to a nitrocellulose membrane, and detected with P2RY5 Antibody at 0.5 ug/mL followed by an HRP-conjugated secondary antibody and ECL detection. A specific band is observed at approximately 39 kDa, consistent with the predicted molecular weight of LPAR6/P2RY5. These results demonstrate that P2RY5 Antibody, a Lysophosphatidic Acid Receptor Antibody, specifically detects endogenous receptor expression in multiple human cell lines.

P2RY5 Antibody PC-3 Cell FACS. Flow cytometric analysis of fixed PC-3 cells using P2RY5 Antibody. Cells were fixed with 4% paraformaldehyde, blocked with normal goat serum, and incubated with P2RY5 Antibody at 1 ug per 1 × 10^6 cells for 30 minutes. A Fluoro488-conjugated goat anti-rabbit secondary antibody was used for detection. Red: unstained cells. Green: rabbit IgG isotype control. Blue: P2RY5 Antibody. The clear rightward shift of the blue histogram relative to the isotype control demonstrates specific detection of endogenous LPAR6/P2RY5 expression in human PC-3 cells. These results show that P2RY5 Antibody, a Lysophosphatidic Acid Receptor Antibody, is suitable for flow cytometric analysis of receptor expression.