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Home >> Antibodies >> p27KIP1 Polyclonal Antibody

p27KIP1 Polyclonal Antibody (R31499)

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  Catalog No Formulation Size Price (USD)  
Image R31499 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 449
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p27KIP1 Polyclonal Antibody WB. Western blot testing of p27KIP1 antibody and Lane 1: rat thymus; 2: rat brain lysate. Expected/observed molecular weight ~27 kDa.
p27KIP1 Polyclonal Antibody FACS. Flow cytometry testing of human SiHa cells with p27KIP1 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= p27KIP1 antibody.
IHC staining of FFPE human tonsil with p27KIP1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human tonsil with p27KIP1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer with p27KIP1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
p27KIP1 Polyclonal Antibody Immunohistochemistry. IHC staining of FFPE rat brain with p27KIP1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
p27KIP1 Polyclonal Antibody Immunocytochemistry. ICC staining of FFPE human U-2 OS cells with p27KIP1 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Availability 1-3 business days
Species Reactivity Human, Rat
Format Antigen affinity purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Gene ID 1027
Applications Western blot: 0.5-1ug/ml
Immunohistochemistry (FFPE): 1-2ug/ml
Flow cytometry: 1-3ug/10^6 cells
Immunocytochemistry (FFPE): 2-4ug/ml
Limitations This p27KIP1 Polyclonal Antibody is available for research use only.
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Description

p27KIP1 Polyclonal Antibody detects Cyclin-dependent kinase inhibitor 1B, also known as KIP1 or P27, an enzyme inhibitor that in humans is encoded by the CDKN1B gene. It encodes a protein which belongs to the Cip/Kip family of cyclin dependent kinase (Cdk) inhibitor proteins. p27KIP1 can inhibit both CDK activation and the kinase activity of assembled and activated cyclin-CDK. The function of the protein is associated with an aggressive phenotype in human breast cancer. Downregulation of p27KIP1 by CK2-alpha-prime is necessary for development of agonist- and stress-induced cardiac hypertrophy.

For additional research options, explore our highly published p27Kip1 Antibody SX53G8, a well-established monoclonal antibody targeting CDKN1B.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the p27KIP1 Polyclonal Antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human partial recombinant protein (AA 10-198) was used as the immunogen for this p27KIP1 antibody.

Storage

After reconstitution, the p27KIP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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