p27Kip1 Antibody KIP1/769 / p21 Mouse MAb [clone KIP1/769] (V2436)

Description

p27Kip1 Antibody KIP1/769 recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This mAb is excellent for staining of formalin-fixed tissues.

For additional research options, explore our highly published p27Kip1 Antibody SX53G8, a well-established monoclonal antibody targeting CDKN1B.

Application Notes

Optimal dilution of the p27Kip1 Antibody KIP1/769 to be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human protein was used as the immunogen for the p27Kip1 antibody.

Storage

Store the p27Kip1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).