- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
p27Kip1 Antibody SX53G8 CF488 / CDKN1B Flow Cytometry Antibody [clone SX53G8] (V2086CF488)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V2086CF488-100T | 500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 Tests | 559 |
|
| Species Reactivity | Human, Mouse, Rat |
| Format | CF488 Conjugate |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Clone Name | SX53G8 |
| Purity | Protein G affinity chromatography |
| Localization | Nuclear |
| Applications | Flow cytometry: 5ul per test per one 10^6 cells in 0.1ml or 5ul per 100ul of whole blood Immunofluorescence: 1-2ug/ml |
| Limitations | This p27Kip1 Antibody SX53G8 CF488 / CDKN1B Flow Cytometry Antibody is available for research use only. |
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The p27Kip1 Antibody SX53G8 CF488 / CDKN1B Flow Cytometry Antibody is a fluorescently conjugated monoclonal antibody recognizing cyclin-dependent kinase inhibitor 1B, a member of the Cip/Kip family of cell cycle regulatory proteins. Also known as p27 or KIP1, CDKN1B functions primarily as a negative regulator of cell cycle progression by controlling the activity of cyclin-dependent kinase complexes. Its inhibitory interactions with cyclin E-CDK2 and cyclin A-CDK2 help restrict the transition from G1 to S phase, coordinating cellular proliferation with extracellular growth signals and environmental conditions.
p27 expression is dynamically regulated throughout the cell cycle. Quiescent and growth-arrested cells frequently maintain elevated nuclear p27 levels, whereas proliferating cells generally reduce its abundance to permit CDK activation and DNA replication. This regulation involves phosphorylation, ubiquitination, and proteasomal degradation. Phosphorylation at threonine 187 promotes recognition by the SCF-SKP2 ubiquitin ligase complex, facilitating p27 turnover. Changes in intracellular p27 abundance therefore provide information about the molecular mechanisms governing cell cycle entry, growth arrest, and proliferative activity.
Although predominantly localized to the nucleus, p27 can also accumulate in the cytoplasm, where it participates in processes beyond CDK inhibition. Cytoplasmic p27 has been implicated in the regulation of actin organization, cell migration, and Rho family GTPase signaling. Its intracellular distribution is influenced by phosphorylation and interactions with regulatory proteins, including components of the PI3K-AKT signaling pathway. Alterations in p27 abundance and localization have been documented in multiple malignancies, where reduced nuclear expression or increased cytoplasmic accumulation may be associated with tumor progression and unfavorable clinical outcomes.
Direct fluorescent labeling enables intracellular p27 detection without requiring a fluorophore-conjugated secondary antibody. The CF488 conjugate provides a green fluorescent signal suitable for evaluating p27 expression in fixed and permeabilized cells by flow cytometry. This format supports studies examining differences in intracellular p27 abundance across cell populations, including those undergoing proliferation, differentiation, or growth arrest. NSJ Bioreagents offers this conjugated antibody as an option for investigating CDKN1B regulation in cellular models of cell cycle control and cancer biology.
For additional research options, explore our highly published p27Kip1 Antibody SX53G8, a well-established monoclonal antibody targeting CDKN1B.
Optimal dilution of the p27Kip1 Antibody SX53G8 CF488 / CDKN1B Flow Cytometry Antibody should be determined by the researcher.
Purified GST-p27 fusion protein of human origin was used as the immunogen for this p27Kip1 antibody.
Store the p27K1 antibody at 2-8oC, protected from light.
CDKN1B CF488 antibody, p27 CF488 antibody, KIP1 CF488 antibody, Cyclin-Dependent Kinase Inhibitor 1B antibody
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