- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
P16INK4a Antibody Rabbit Monoclonal / CDKN2A [clone CDKN2A/7660R] (V5280)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V5280-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
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V5280-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
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V5280SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
|
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG, kappa |
| Clone Name | CDKN2A/7660R |
| Purity | Protein A/G affinity |
| UniProt | P42771 |
| Localization | Nucleus, Cytoplasm |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT |
| Limitations | This P16INK4a antibody is available for research use only. |
|
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P16INK4a antibody recognizes Cyclin-dependent kinase inhibitor 2A, the tumor suppressor protein encoded by the CDKN2A gene on chromosome 9p21.3. P16INK4a Antibody Rabbit Monoclonal is developed to detect this critical regulator of cell cycle progression in research applications involving normal and neoplastic tissues. P16INK4a functions primarily in the nucleus, with additional cytoplasmic localization, where it binds to CDK4 and CDK6 to inhibit phosphorylation of the retinoblastoma protein and block transition from G1 to S phase.
CDKN2A antibody, also referred to as P16 antibody and INK4a antibody in the literature, targets a key component of the RB pathway. The CDKN2A locus is unique in that it encodes multiple tumor suppressor proteins through alternative splicing and reading frames, including P14ARF, but P16INK4a specifically acts through inhibition of cyclin D-CDK4 and cyclin D-CDK6 complexes. Loss of CDKN2A function through mutation, deletion, or promoter methylation is frequently observed in melanoma, pancreatic carcinoma, glioma, bladder cancer, and other malignancies.
In normal tissues, P16INK4a expression is generally low but can increase in response to cellular senescence or oncogenic stress. Overexpression of P16INK4a is commonly observed in high-risk human papillomavirus-associated lesions, including cervical intraepithelial neoplasia and carcinoma, where viral oncoproteins inactivate RB, leading to compensatory upregulation of P16. As a result, P16INK4a antibody is widely used in studies investigating cell cycle dysregulation, viral oncogenesis, and tumor suppressor pathway alterations.
Structurally, P16INK4a contains multiple ankyrin repeat motifs that mediate its interaction with CDK4 and CDK6. By preventing formation of active cyclin D-CDK complexes, it maintains RB in a hypophosphorylated state, thereby enforcing cell cycle arrest. Disruption of this regulatory axis promotes uncontrolled proliferation and contributes to tumorigenesis.
The rabbit monoclonal clone CDKN2A/7660R provides specific detection of P16INK4a in formalin-fixed tissues and cell-based systems. Visualization of nuclear and cytoplasmic staining patterns supports research into CDKN2A expression status, cell cycle checkpoint integrity, and mechanisms of malignant transformation at NSJ Bioreagents.
Optimal dilution of the P16INK4a antibody rabbit monoclonal should be determined by the researcher.
Purified recombinant prokaryotic full-length human protein was used as the immunogen for the P16INK4a antibody rabbit monoclonal.
Aliquot the P16INK4a antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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