P15RS Antibody / RPRD1A Antibody (RQ7772)

P15RS Antibody Human A549 Lung Cancer Cell IF. Immunofluorescent staining of human A549 cells was performed with P15RS Antibody at 5 ug/ml and Alpha Tubulin antibody overnight at 4oC. Enzyme antigen retrieval was performed for 15 minutes. P15RS/RPRD1A was detected with Fluoro488-conjugated secondary antibody (green), while Alpha Tubulin was detected with Cy3-conjugated secondary antibody (red). P15RS staining is observed predominantly in the nuclear and perinuclear regions, while Alpha Tubulin highlights the cytoskeletal network. This P15RS Antibody demonstrates localization of RPRD1A in human A549 lung cancer cells.

Western blot testing of human 1) 293T, 2) A431, 3) Jurkat and 4) SH-SY5Y cell lysate with P15RS antibody. Predicted molecular weight ~36 kDa.

Western blot testing of 1) human 293T, 2) human A431, 3) human Jurkat, 4) human SH-SY5Y, 5) rat brain, 6) rat C6, 7) mouse brain and 8) mouse Neuro-2a cell lysate with P15RS antibody. Predicted molecular weight ~36 kDa.

P15RS Antibody Human, Mouse and Rat WB. Western blot analysis was performed with P15RS Antibody on human 293T, A431, Jurkat and SH-SY5Y cell lysates (lanes 1-4), rat brain and C6 cell lysates (lanes 5-6), and mouse brain tissue lysate (lane 7). Samples (30 ug) were separated by 10% SDS-PAGE under reducing conditions, and the antibody was used at 0.25 ug/ml overnight at 4oC. A prominent band was detected at approximately 36 kDa, consistent with the expected molecular weight of RPRD1A. This P15RS Antibody demonstrates detection of RPRD1A across human, mouse and rat samples.

P15RS Antibody Human Rectal Adenocarcinoma IHC. IHC staining of FFPE human rectal adenocarcinoma tissue was performed with P15RS Antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to staining. Positive RPRD1A staining is observed predominantly in the nuclei of malignant glandular epithelial cells. This P15RS Antibody demonstrates nuclear localization of RPRD1A in human rectal adenocarcinoma tissue.

P15RS Antibody Rat Brain Hippocampus IHC. IHC staining of FFPE rat brain tissue was performed with P15RS Antibody at 2 ug/ml overnight at 4oC. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer, followed by HRP-conjugated secondary antibody and DAB detection. Positive RPRD1A staining is observed prominently in nuclei within the hippocampal neuronal layers. This P15RS Antibody demonstrates nuclear localization of RPRD1A in rat brain tissue.

P15RS Antibody Mouse Brain Nuclear IHC. IHC staining of FFPE mouse brain tissue was performed with P15RS Antibody at 2 ug/ml overnight at 4oC. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer, followed by HRP-conjugated secondary antibody and DAB detection. Positive RPRD1A staining is observed prominently in the nuclei of cells throughout the brain tissue. This P15RS Antibody demonstrates nuclear localization of RPRD1A in mouse brain tissue.

P15RS Antibody Human Breast Cancer IHC. IHC staining of FFPE human breast cancer tissue was performed with P15RS Antibody at 2 ug/ml overnight at 4oC. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer, followed by HRP-conjugated secondary antibody and DAB detection. Positive RPRD1A staining is observed in the tumor cells with prominent nuclear and cytoplasmic signal. This P15RS Antibody demonstrates localization of RPRD1A in human breast cancer tissue.

P15RS Antibody Human Colon Cancer IHC. IHC staining of FFPE human colon cancer tissue was performed with P15RS Antibody at 2 ug/ml overnight at 4oC. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer, followed by HRP-conjugated secondary antibody and DAB detection. Positive RPRD1A staining is observed in the malignant glandular epithelial cells with prominent nuclear and cytoplasmic signal. This P15RS Antibody demonstrates localization of RPRD1A in human colon cancer tissue.

P15RS Antibody Human Colorectal Adenocarcinoma IHC. IHC staining of FFPE human colon cancer tissue was performed with P15RS Antibody at 2 ug/ml overnight at 4oC. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer, followed by HRP-conjugated secondary antibody and DAB detection. Positive RPRD1A staining is observed in malignant glandular epithelial cells with prominent nuclear and cytoplasmic signal. This P15RS Antibody demonstrates localization of RPRD1A in human colorectal adenocarcinoma tissue.

P15RS Antibody Human SH-SY5Y Neuroblastoma Cell IP. Immunoprecipitation of P15RS/RPRD1A from human SH-SY5Y neuroblastoma whole cell lysate was performed with P15RS Antibody. Input lysate (lane 1), rabbit control IgG IP (lane 2) and P15RS Antibody IP (lane 3) were analyzed by western blot. A specific RPRD1A band is detected at approximately 36 kDa in the input and antibody immunoprecipitate, consistent with the expected molecular weight. The prominent approximately 50 kDa signal represents IgG heavy chain. This P15RS Antibody demonstrates immunoprecipitation of RPRD1A from human SH-SY5Y neuroblastoma cells.