- Tel: 858.663.9055
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
p-Tyr Antibody / Phosphotyrosine [clone SPM102] (V7502)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
 |
V7502-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 519 | |
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V7502-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 229 | |
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V7502SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 519 |
| Availability | 1-3 business days |
| Species Reactivity | NA |
| Format | Purified |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2b, kappa |
| Clone Name | SPM102 |
| Purity | Protein G affinity chromatography |
| Localization | Depends upon the location of phosphorylated target |
| Applications | Flow cytometry : 1-2ug/10^6 cells Immunofluorescence : 1-2ug/ml Western blot : 1-2ug/ml Immunoprecipitation : 1-2ug/500ug protein lysate Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT |
| Limitations | This p-Tyr antibody is available for research use only. |
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Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This mAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.
Optimal dilution of the p-Tyr antibody should be determined by the researcher.
1. No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues
Phosphotyrosine conjugated to BSA was used as the immunogen for the p-Tyr antibody.
Store the p-Tyr antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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