P-Cadherin Antibody / Epithelial Cell Adhesion Antibody (RQ6762)

P-Cadherin Antibody A431 Cell IF. Immunofluorescence analysis of FFPE human A431 cells using P-Cadherin Antibody / Epithelial Cell Adhesion Antibody. Heat induced antigen retrieval was performed by steaming sections in pH 6 citrate buffer for 20 minutes. P-Cadherin expression is shown in green with DAPI nuclear counterstain shown in blue. Strong membrane-associated staining is observed at cell-cell junctions, supporting the use of P-Cadherin Antibody / Epithelial Cell Adhesion Antibody for studying epithelial adhesion proteins, adherens junction formation and cell communication pathways.

P-Cadherin Antibody A431 Cell WB. Western blot analysis of human A431 cell lysate using P-Cadherin Antibody / Epithelial Cell Adhesion Antibody. A specific band is detected between approximately 100–130 kDa, consistent with the expected migration range of glycosylated P-Cadherin. The unmodified protein has a predicted molecular weight of approximately 91 kDa. This result supports the use of P-Cadherin Antibody / Epithelial Cell Adhesion Antibody for analyzing epithelial adhesion protein expression and cadherin family signaling pathways in human cancer cell models.

P-Cadherin Antibody Human A431 Cell WB. Western blot analysis of human A431 cell lysate using P-Cadherin Antibody / Epithelial Cell Adhesion Antibody. A specific band is observed between approximately 100–130 kDa, consistent with the expected molecular weight range of glycosylated P-Cadherin. The unmodified CDH3 protein has a predicted molecular weight of approximately 91 kDa. These results support the use of P-Cadherin Antibody / Epithelial Cell Adhesion Antibody for studying epithelial cell adhesion proteins, cadherin expression and cell junction regulation.

P-Cadherin Antibody A431 Cell FACS. Flow cytometry analysis of fixed and permeabilized human A431 cells using P-Cadherin Antibody / Epithelial Cell Adhesion Antibody. Cells were blocked with goat serum and incubated with the primary antibody at 1 ug/million cells. The blue histogram represents P-Cadherin staining, while green represents isotype control and red represents unstained cells. Clear positive signal separation supports the use of P-Cadherin Antibody / Epithelial Cell Adhesion Antibody for analyzing epithelial adhesion protein expression and cadherin-mediated cell communication pathways by flow cytometry.