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Home >> Antibodies >> Osteopontin Antibody / OPN / SPP1

Osteopontin Antibody / OPN / SPP1 [clone CEO-19] (RQ5325)

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Image RQ5325 Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA 100 ul 429
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Western blot testing of human HepG2 cell lysate with Osteopontin antibody. Predicted molecular weight: 35-65 kDa depending on degree of glycosylation.
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Western blot testing of human HepG2 cell lysate with Osteopontin antibody. Predicted molecular weight: 35-65 kDa depending on degree of glycosylation.
Western blot testing of human 1) HepG2, 2) SW-480, 3) SK-O-V3, 4) MCF7 and 5) HeLa cell lysate with Osteopontin antibody. Predicted molecular weight: 35-65 kDa depending on degree of glycosylation.
Immunofluorescent staining of FFPE human HeLa cells with Osteopontin antibody (1:50 dilution, green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human HeLa cells with Osteopontin antibody (1:150 dilution, green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Availability 1-2 weeks
Species Reactivity Human
Format Purified
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name CEO-19
Purity Affinity purified
UniProt P10451
Applications Western blot : 1:500-1:2000
Immunofluorescence : 1:50-1:200
Limitations This Osteopontin antibody is available for research use only.
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Description

The protein encoded by the SPP1 gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [RefSeq]

Application Notes

Optimal dilution of the Osteopontin antibody should be determined by the researcher.

Immunogen

A synthetic peptide specific to human Osteopontin / SPP1 was used as the immunogen for the Osteopontin antibody.

Storage

Store the Osteopontin antibody at -20oC.

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