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Home >> Antibodies >> OS9 Antibody / Amplified in Osteosarcoma 9

OS9 Antibody / Amplified in Osteosarcoma 9 (RQ6667)

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  Catalog No Formulation Size Price (USD)  
Image RQ6667 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human SiHa cells with OS9 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human SiHa cells with OS9 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human 293T, 3) human HL60, 4) rat pancreas and 5) mouse pancreas tissue lysate with OS9 antibody. Expected molecular weight: 61-76 kDa (multiple isoforms) but may be observed at higher molecular weights due to glycosylation. Isoform OS-9-1 is commonly observed at 76-97 kDa and isoform OS-9-2 is commonly observed at 69-83 kDa.
Availability 1-3 business days
Species Reactivity Human, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q13438
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This OS9 antibody is available for research use only.
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Description

Protein OS-9 is a protein that in humans is encoded by the OS9 gene. This gene encodes a protein that is highly expressed in osteosarcomas. This protein binds to the hypoxia-inducible factor 1 (HIF-1), a key regulator of the hypoxic response and angiogenesis, and promotes the degradation of one of its subunits. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Application Notes

Optimal dilution of the OS9 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids Q56-E635) was used as the immunogen for the OS9 antibody.

Storage

After reconstitution, the OS9 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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