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Home >> Antibodies >> Ornithine Aminotransferase Antibody / OAT

Ornithine Aminotransferase Antibody / OAT (RQ6502)

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  Catalog No Formulation Size Price (USD)  
Image RQ6502 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human lung cancer tissue with Ornithine Aminotransferase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human lung cancer tissue with Ornithine Aminotransferase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer tissue with Ornithine Aminotransferase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human testicular cancer tissue with Ornithine Aminotransferase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) K562, 2) A431, 3) HEK293, 4) Caco-2, 5) U-87 MG, 6) HepG2, 7) U-2 OS, 8) rat kidney, 9) rat testis, 10) mouse brain and 11) mouse kidney lysate with Ornithine Aminotransferase antibody. Predicted molecular weight ~49 kDa.
Flow cytometry testing of human A549 cells with Ornithine Aminotransferase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ornithine Aminotransferase antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P04181
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Ornithine Aminotransferase antibody is available for research use only.
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Description

Ornithine aminotransferase (OAT) is an enzyme which is encoded in human by the OAT gene located on chromosome 10. This gene encodes the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. Alternatively spliced transcript variants encoding different isoforms have been described. Related pseudogenes have been defined on the X chromosome.

Application Notes

Optimal dilution of the Ornithine Aminotransferase antibody should be determined by the researcher.

Immunogen

An E. coli-derived human protein (amino acids A214-F439) was used as the immunogen for the Ornithine Aminotransferase antibody.

Storage

After reconstitution, the Ornithine Aminotransferase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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