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Home >> Antibodies >> ONECUT1 Antibody / One cut homeobox 1 / HNF6

ONECUT1 Antibody / One cut homeobox 1 / HNF6 (RQ7950)

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  Catalog No Formulation Size Price (USD)  
Image RQ7950 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human U-2 OS cells with ONECUT1 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human U-2 OS cells with ONECUT1 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HepG2 and 2) MCF7 cell lysate with ONCECUT1 antibody. Predicted molecular weight ~51 kDa.
Flow cytometry testing of human Daudi cells with ONECUT1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ONECUT1 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9UBC0
Localization Nuclear
Applications Western blot : 0.5-1ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This ONECUT1 antibody is available for research use only.
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Description

One cut homeobox 1 (ONECUT1), also called Hepatocyte nuclear factor 6 (HNF6), is found strong expression in liver and lower expression in testis and skin. ONECUT1 gene is mapped to 15q21.3. This gene encodes a member of the Cut homeobox family of transcription factors. Expression of the encoded protein is enriched in the liver, where it stimulates transcription of liver-expressed genes, and antagonizes glucocorticoid-stimulated gene transcription. This gene may influence a variety of cellular processes including glucose metabolism, cell cycle regulation, and it may also be associated with cancer.

Application Notes

Optimal dilution of the ONECUT1 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids M1-E270) was used as the immunogen for the ONECUT1 antibody.

Storage

After reconstitution, the ONECUT1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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