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Home >> Antibodies >> Oct4 Antibody / POU5F1

Oct4 Antibody / POU5F1 [clone Oct4/3508] (V9371)

  Catalog No Formulation Size Price (USD)  
Image V9371-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9371-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9371SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human seminoma with Oct4 antibody (clone Oct4/3508). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human seminoma with Oct4 antibody (clone Oct4/3508). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name Oct4/3508
Purity Protein A/G affinity
UniProt Q01860
Localization Nucleus
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This Oct4 antibody is available for research use only.
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Description

Oct4 Transcription factor that binds to the octamer motif (5). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. Interacts with UBE2I and ZSCAN10. Interacts with PKM2. Interacts with WWP2. Transcriptional activity is positively regulated by PKM2. Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. Belongs to the POU transcription factor family. Class- 5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Application Notes

Optimal dilution of the Oct4 antibody should be determined by the researcher.

Immunogen

A portion of amino acids 1-134 was used as the immunogen for the Oct4. antibody.

Storage

Aliquot the Oct4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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