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Home >> Antibodies >> OCLN Antibody / Occludin

OCLN Antibody / Occludin [clone MSVA-415M] (V6057)

  Catalog No Formulation Size Price (USD)  
Image V6057-100UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 654.50
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V6057-20UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 324.50
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Occludin Mouse Monoclonal Antibody (MSVA-415M) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Species Reactivity Human
Format Purified
Host Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name MSVA-415M
Purity Protein G affinity
UniProt Q16625
Localization Cell junction, Cell membrane, Tight junction
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This OCLN/Occludin antibody is available for research use only.
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Application Notes

Optimal dilution of the OCLN/Occludin antibody should be determined by the researcher.

Immunogen

A recombinant fragment of human Occludin protein (around amino acids 282-415) (exact sequence is proprietary) was used as the immuongen for the OCLN/Occludin antibody.

Storage

OCLN/Occludin antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

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