Occludin Antibody Microarray Validated / OCLN [clone OCLN/2183] (V7647)

Immunohistochemistry of Occludin antibody in human kidney tissue. The microarray validated clone OCLN/2183 demonstrates strong membranous HRP-DAB brown staining along renal tubular epithelial cell borders, consistent with tight junction localization of Occludin. Staining highlights apical lateral cell-cell junctions forming continuous circumferential patterns within tubular structures, while surrounding interstitial tissue shows minimal background signal. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes followed by cooling prior to incubation.

IHC staining of FFPE human kidney with Occludin antibody (clone OCLN/2183). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

HuProt human protein microarray specificity analysis of Occludin antibody clone OCLN/2183. The microarray validated monoclonal antibody was screened against more than 19,000 full-length human proteins. OCLN ranked as the top hit with the highest signal intensity, demonstrating strong target specificity relative to other proteins on the array.
Z-score represents the strength of the signal generated when the antibody, together with a fluorescently labeled anti-IgG secondary antibody, binds to a specific protein on the HuProt array. Z-scores are expressed in units of standard deviations above the mean signal of all proteins on the array. When proteins are ranked in descending order by Z-score, the S-score reflects the difference in standard deviations between adjacent ranked proteins. The S-score therefore indicates the relative specificity of the antibody for its intended target compared with other proteins on the array.

SDS-PAGE analysis of purified, BSA-free Occludin antibody (clone OCLN/2183) as confirmation of integrity and purity.