Oat3 Antibody / Slc22a8 (RQ6266)

Oat3 Antibody / Slc22a8 Neuro-2a FACS. Flow cytometry analysis of Neuro-2a cells using Oat3 Antibody / Slc22a8 Antibody demonstrates a strong positive fluorescence shift relative to both unstained cells and isotype control-treated cells, consistent with expression of Organic Anion Transporter 3 (OAT3/Slc22a8). Overlay histograms compare cells alone (red), isotype control antibody (green), and Oat3 antibody (blue), with the specific antibody producing a pronounced rightward shift indicative of target-associated binding. Cells were blocked with goat serum prior to staining, and antibody was used at 1 μg per million cells. These results support application of the antibody for flow cytometric detection of OAT3 expression and transporter-associated cellular studies.

Oat3 Antibody / Slc22a8 Mouse Kidney WB. Western blot analysis of mouse kidney lysate using Oat3 Antibody / Slc22a8 Antibody demonstrates a distinct band at approximately 60 kDa, consistent with the predicted molecular weight of Organic Anion Transporter 3 (OAT3/Slc22a8). OAT3 is a membrane-associated transporter involved in the uptake and transport of organic anions, endogenous metabolites, pharmaceutical compounds, and xenobiotic substances. The observed band supports expression of OAT3 in renal tissue and validates the antibody for Western blot applications investigating kidney physiology, transporter biology, and drug transport mechanisms.