• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Order
Home >> Antibodies >> O-GlcNAcase Antibody / OGA / MGEA5

O-GlcNAcase Antibody / OGA / MGEA5 (RQ6503)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image RQ6503 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
Bulk quote request
Immunofluorescent staining of FFPE human T-47D cells with O-GlcNAcase antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
›
Immunofluorescent staining of FFPE human T-47D cells with O-GlcNAcase antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE rat spleen tissue with O-GlcNAcase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HEK293, 2) human HeLa, 3) rat brain and 4) mouse brain lysate with O-GlcNAcase antibody. Predicted molecular weight ~103 kDa, routinely observed at ~130 kDa (cytoplasmic form) and ~75 kDa (nuclear form).
Flow cytometry testing of human U-87 MG cells with O-GlcNAcase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= O-GlcNAcase antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt O60502
Localization Cytoplasmic, nuclear
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This O-GlcNAcase antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

Description

Protein O-GlcNAcase (OGA) is an enzyme with systematic name (protein)-3-O-(N-acetyl-D-glucosaminyl)-L-serine/threonine N-acetylglucosaminyl hydrolase. The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT, which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications.

Application Notes

Optimal dilution of the O-GlcNAcase antibody should be determined by the researcher.

Immunogen

Amino acids KVYTIRPYFPKDEASVYKICRE from the human protein were used as the immunogen for the O-GlcNAcase antibody.

Storage

After reconstitution, the O-GlcNAcase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Related Products

   1.   View all MGEA5 antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.