NUP62 Antibody / Nucleoporin 62 Antibody (FY12565)

NUP62 Antibody in Human U2OS Osteosarcoma Cells IF. Immunofluorescence staining of human U2OS osteosarcoma cells with NUP62 Antibody / Nucleoporin 62 Antibody (red) at 5 ug/ml. Enzyme-mediated antigen retrieval was performed for 15 min, followed by blocking with 10% goat serum. Cells were incubated with the primary antibody overnight at 4oC. Cy3-conjugated goat anti-rabbit IgG was used as the secondary antibody at 1:500 for 30 min at 37oC, and nuclei were counterstained with DAPI (blue). NUP62 staining is concentrated in the nuclear region. This NUP62 Antibody enables visualization of Nucleoporin 62 expression and localization in human osteosarcoma cells.

NUP62 Antibody in Human and Mouse Samples WB. Western blot testing of NUP62 Antibody / Nucleoporin 62 Antibody using lysates from 1) mouse testis, 2) human Jurkat cells, 3) human 293T cells, 4) human HeLa cells and 5) human PC-3 cells. The antibody was used at 0.5 ug/ml overnight at 4oC, followed by goat anti-rabbit IgG-HRP at 1:5000 and chemiluminescent detection. A strong band is detected at approximately 69 kDa in the human cell lines, consistent with the known slower SDS-PAGE migration of NUP62 relative to its calculated molecular weight of approximately 53 kDa. Mouse testis shows a ~69 kDa doublet. This NUP62 Antibody demonstrates detection of Nucleoporin 62 in human and mouse samples.

NUP62 Antibody and Beta Tubulin in Human U2OS Cells IF. Immunofluorescence staining of human U2OS osteosarcoma cells with NUP62 Antibody / Nucleoporin 62 Antibody (red) at 5 ug/ml and Beta Tubulin Antibody (green). Enzyme-mediated antigen retrieval was performed for 15 min, followed by blocking with 10% goat serum. Cells were incubated with the primary antibodies overnight at 4oC. Cy3-conjugated goat anti-rabbit IgG and DyLight 488-conjugated goat anti-mouse IgG were used as secondary antibodies at 1:500 for 30 min at 37oC. NUP62 staining is concentrated in the nuclear region, while Beta Tubulin highlights the cytoskeletal network. This NUP62 Antibody enables visualization of Nucleoporin 62 localization in human osteosarcoma cells.

NUP62 Antibody in Human Ovarian Cancer IHC. Immunohistochemistry staining of FFPE human ovarian cancer tissue with NUP62 Antibody / Nucleoporin 62 Antibody at 2 ug/ml. Heat-induced epitope retrieval (HIER) was performed in EDTA buffer, pH 8.0, followed by blocking with 10% goat serum. The tissue was incubated with the primary antibody overnight at 4oC, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody for 30 min at 37oC and DAB detection. NUP62 staining is observed in the tumor cells. This NUP62 Antibody demonstrates detection of Nucleoporin 62 in human ovarian cancer tissue.

NUP62 Antibody in Human PC-3 Prostate Cancer Cells FACS. Flow cytometry analysis of fixed and permeabilized human PC-3 prostate cancer cells with NUP62 Antibody / Nucleoporin 62 Antibody at 1 ug/million cells. Cells were blocked with 10% normal goat serum and incubated with the primary antibody for 30 min at 20oC. DyLight 488-conjugated goat anti-rabbit IgG was used as the secondary antibody at 5-10 ug/million cells for 30 min at 20oC. Red represents the unlabeled control, green represents the isotype control and blue represents NUP62 Antibody staining. A clear fluorescence shift is observed with NUP62 staining relative to the isotype control. This NUP62 Antibody demonstrates detection of Nucleoporin 62 in human prostate cancer cells by flow cytometry.