NUP35 Antibody / Nucleoporin NUP35 Antibody (FY13539)

NUP35 Antibody by IF in A549 cells. Immunofluorescence analysis of human A549 cells using Nucleoporin NUP35 Antibody demonstrates green staining concentrated around the nuclear periphery, forming a ring-like pattern consistent with localization at the nuclear envelope. Cells underwent enzyme-mediated antigen retrieval for 15 minutes and blocking with 10% goat serum. Primary antibody was incubated at 5 ug/mL overnight at 4oC, followed by DyLight 488-conjugated secondary antibody at 1:500 for 30 minutes at 37oC. The distribution is consistent with the nuclear pore-associated function of NUP35.

NUP35 Antibody by WB in human and rodent lysates. Western blot analysis using Nucleoporin NUP35 Antibody identifies a band near 35 kDa in human 293T (lane 1), K562 (lane 2), HeLa (lane 3), HepG2 (lane 4), rat testis (lane 5), rat RH35 cells (lane 6), mouse testis (lane 7), and NIH/3T3 cells (lane 8). The observed migration agrees with the expected approximately 35 kDa molecular weight of NUP35. Reduced lysates containing 30 ug protein per lane were separated by 10% SDS-PAGE and transferred to nitrocellulose. Primary antibody was incubated at 0.5 ug/mL overnight at 4oC and detected using an HRP-conjugated secondary antibody and chemiluminescence.

NUP35 Antibody by FACS in K562 cells. Flow cytometric analysis using Nucleoporin NUP35 Antibody shows a rightward fluorescence shift in stained K562 cells (blue histogram) relative to the rabbit IgG isotype control (green) and unstained cells (red). Cells were fixed in 4% paraformaldehyde and permeabilized to permit intracellular antigen detection, then blocked with 10% goat serum. Primary antibody was applied at 1 ug per 1 x 10^6 cells for 30 minutes at 20oC, followed by DyLight 488-conjugated secondary antibody. The histogram documents intracellular antibody-associated fluorescence.