Nucleostemin Antibody / GNL3 (RQ6638)

IHC staining of FFPE human breast cancer tissue with Nucleostemin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human appendiceal adenocarcinoma tissue with Nucleostemin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colonic adenocarcinoma tissue with Nucleostemin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human gall bladder adenosquamous carcinoma tissue with Nucleostemin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human Hep3B cells with Nucleostemin antibody (Dylight 488-conjugated secondary, green), Phalloidin-iFluor 555 conjugate (red) and DAPI (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of 1) human HeLa, 2) human K562, 3) human PC-3, 4) human Jurkat, 5) rat C6 and 6) mouse NIH 3T3 cell lysate with Nucleostemin antibody. Predicted molecular weight: ~62/60 kDa (isoforms 1/2).

Flow cytometry testing of human HeLa cells with Nucleostemin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Nucleostemin antibody.