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Home >> Antibodies >> Nucleoside diphosphate kinase B Antibody / NDKB / NME2

Nucleoside diphosphate kinase B Antibody / NDKB / NME2 [clone NME2/12061] (V5770)

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  Catalog No Formulation Size Price (USD)  
Image V5770-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5770-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5770SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
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IHC staining of FFPE human kidney tissue with Nucleoside diphosphate kinase B antibody (clone NME2/12061). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human kidney tissue with Nucleoside diphosphate kinase B antibody (clone NME2/12061). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Nucleoside diphosphate kinase B antibody (clone NME2/12061) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG, kappa
Clone Name NME2/12061
Purity Protein A/G affinity
UniProt P22392
Localization Cytoplasm, Nucleus
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This Nucleoside diphosphate kinase B antibody is available for research use only.
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Description

The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nuclease hypersensitive elements at positions 142 to 115 of the human c-Myc promotor.

Application Notes

Optimal dilution of the Nucleoside diphosphate kinase B antibody should be determined by the researcher.

Immunogen

A recombinant full-length human NME2 protein was used as the immunogen for the Nucleoside diphosphate kinase B antibody.

Storage

Aliquot the Nucleoside diphosphate kinase B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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