Nucleoporin 107 Antibody / Nuclear Pore Complex Antibody (RQ8307)

Nucleoporin 107 Antibody in Human U-2 OS Osteosarcoma Cells IF. Immunofluorescence staining of FFPE human U-2 OS osteosarcoma cells with Nucleoporin 107 Antibody / Nuclear Pore Complex Antibody (green) and Phalloidin (red). Heat-induced epitope retrieval (HIER) was performed by steaming in pH 6 citrate buffer for 20 min. NUP107 staining is concentrated in the nuclear region, consistent with its association with the nuclear pore complex. This Nucleoporin 107 Antibody enables visualization of NUP107 expression and localization in human osteosarcoma cells.

Nucleoporin 107 Antibody in Human, Rat and Mouse Samples WB. Western blot testing of Nucleoporin 107 Antibody / Nuclear Pore Complex Antibody using lysates from 1) human COLO-320 cells, 2) human HepG2 cells, 3) human 293T cells, 4) human HeLa cells, 5) human PC-3 cells, 6) human K562 cells, 7) human HEL cells, 8) rat PC-12 cells, 9) mouse testis and 10) mouse NIH 3T3 cells. A prominent band is detected near the predicted molecular weight of approximately 107 kDa. This Nucleoporin 107 Antibody demonstrates detection of NUP107 in human, rat and mouse samples.

Nucleoporin 107 Antibody in Human HEL Erythroleukemia Cells FACS. Flow cytometry testing of fixed and permeabilized human HEL erythroleukemia cells with Nucleoporin 107 Antibody / Nuclear Pore Complex Antibody at 1 ug/million cells following blocking with goat sera. Red represents cells alone, green represents the isotype control and blue represents Nucleoporin 107 Antibody staining. A clear fluorescence shift is observed with antibody staining relative to the isotype control. This Nucleoporin 107 Antibody demonstrates detection of NUP107 in human HEL cells by flow cytometry.

Nucleoporin 107 Antibody in Human 293T Cells IP. Immunoprecipitation of Nucleoporin 107 protein from 500 ug of human 293T whole cell lysate was performed using 2 ug of Nucleoporin 107 Antibody / Nuclear Pore Complex Antibody. The immunoprecipitated sample was analyzed by western blot, with NUP107 detected near its expected molecular weight of approximately 107 kDa. This Nucleoporin 107 Antibody demonstrates immunoprecipitation of endogenous NUP107 from human 293T cells.

Nucleoporin 107 Antibody in Human Liver Cancer IHC. Immunohistochemistry analysis of FFPE human liver cancer tissue with Nucleoporin 107 Antibody / Nuclear Pore Complex Antibody at 2 ug/ml. Heat-induced epitope retrieval (HIER) was performed in EDTA buffer, pH 8.0, followed by blocking with 10% goat serum. The tissue was incubated with the primary antibody overnight at 4oC, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody for 30 min at 37oC and DAB detection. NUP107 staining is observed in the tumor cells. This Nucleoporin 107 Antibody demonstrates detection of NUP107 in human liver cancer tissue.