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Home >> Antibodies >> Nucleophosmin Antibody

Nucleophosmin Antibody (R31754)

  Catalog No Formulation Size Price (USD)  
Image R31754 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 329
Western blot testing of Nucleophosmin antibody and Lane 1: rat testis; 2: mouse testis; 3: human SKOV; 4: (m) HEPA; 5: (h) Jurkat; 6: (h) HUT; 7: (h) HeLa. Expected/observed molecular weight: ~38kDa.
IHC-P: Nucleophosmin antibody testing of human intestine cancer tissue
IHC-P: Nucleophosmin antibody testing of mouse intestine tissue
IHC-P: Nucleophosmin antibody testing of rat intestine tissue
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Gene ID 4869
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
Limitations This Nucleophosmin antibody is available for research use only.
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Description

NPM1 or NPM (Nucleophosmin/Nucleoplasmin family member1), also known as Nucleolar phosphoprotein B23 or numatrin, is a protein that in humans is encoded by the NPM1 gene. Chan et al. (1989) found that NPM is a nucleolar phosphoprotein that is more abundant in tumor cells than in normal resting cells. Stimulation of the growth of normal cells, e.g., mitogen activation of B lymphocytes, was accompanied by an increase in Nucleophosmin protein level. They stated that the protein is likely involved in the assembly of ribosomal proteins into ribosomes. Electron microscopic study indicated that Nucleophosmin is concentrated in the granular region of the nucleolus, where ribosome assembly occurs.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the Nucleophosmin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human partial recombinant protein (AA 1-294) was used as the immunogen for this Nucleophosmin antibody.

Storage

After reconstitution, the Nucleophosmin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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