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Home >> Antibodies >> Nucleophosmin Antibody (NPM1)

Nucleophosmin Antibody (NPM1) (R31031)

  Catalog No Formulation Size Price (USD)  
Image R31031 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human A431 cells with Nucleophosmin antibody. HIER: steam section in pH6 citrate buffer for 20 min.
IHC-P: Nucleophosmin antibody testing of human intestinal cancer tissue. HIER: steamed with pH6 citrate buffer.
ICC testing of Nucleophosmin antibody and HeLa cells
IHC-P: Nucleophosmin antibody testing of rat spleen tissue. HIER: steamed with pH6 citrate buffer.
Western blot testing of Nucleophosmin antibody and Lane 1: rat testis; 2: rat brain; 3: human HeLa; 4: human U87 MG; 5: human A549; 6: human SMMC-7721 cell lysate. Expected molecular weight: ~38 kDa.
Availability 1-3 business days
Species Reactivity Human, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P06748
Localization Nucleus, cytoplasm
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml
Immunocytochemistry : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Limitations This Nucleophosmin antibody is available for research use only.
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Description

Nucleophosmin, also known as NPM, is a protein that in humans is encoded by the NPM1 gene. The gene maps to chromosome 5q35. Nucleophosmin is likely involved in the assembly of ribosomal proteins into ribosomes. An electron microscopic study indicated that Nucleophosmin is concentrated in the granular region of the nucleolus, where ribosome assembly occurs.

Application Notes

Variations in secondary/substrate sensitivities and test protocols may require the Nucleophosmin antibody to be titrated for optimal performance.

Immunogen

An amino acid sequence from the N-terminus of human NPM1 (ELKADKDYHFKVDNDENEHQ) was used as the immunogen for this Nucleophosmin antibody (100% homologous in human, mouse and rat).

Storage

The lyophilized Nucleophosmin antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freeze/thaws.

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