Nucleophosmin Antibody / NPM1 [clone NPM1/3287] (V7550)

Western blot testing of human K562 cell lysate with Nucleophosmin antibody (clone NPM1/3287). Expected molecular weight: ~38 kDa.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Nucleophosmin antibody (clone NPM1/3287). These results demonstrate the foremost specificity of the NPM1/3287 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Nucleophosmin antibody (clone NPM1/3287) as confirmation of integrity and purity.

IHC staining of FFPE human basal cell carcinoma with Nucleophosmin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Immunofluorescent staining of human HeLa cells with Nucleophosmin antibody (clone NPM1/3287, green) and phalloidin (red).

Flow cytometry staining of PFA-fixed human HeLa cells with Nucleophosmin antibody; Red=isotype control, Blue= Nucleophosmin antibody.