Nucleolin Antibody Rabbit Polyclonal (RQ5539)

Western blot testing of human HepG2 cell lysate with Nucleolin antibody. Predicted molecular weight ~77 kDa, commonly observed at 100~110 kDa.

Nucleolin Antibody Rabbit Polyclonal western blot analysis. Lane 1: human K562 lysate, Lane 2: human HeLa lysate, Lane 3: monkey COS-7 lysate. A band is detected at approximately 100-110 kDa, consistent with the commonly observed migration of Nucleolin / NCL despite a predicted molecular weight of approximately 77 kDa. The higher apparent size is well documented for this nucleolar phosphoprotein and reflects extensive post-translational modification and acidic domain composition that alter SDS-PAGE migration.

Nucleolin Antibody Rabbit Polyclonal immunohistochemistry analysis of human renal pelvis tissue. Formalin-fixed paraffin-embedded human squamous metaplasia of the renal pelvis stained with Nucleolin Antibody Rabbit Polyclonal. HRP-DAB brown chromogenic staining highlights nuclei of epithelial cells within the metaplastic squamous epithelium, consistent with the expected nuclear and nucleolar localization of Nucleolin / NCL. HIER: boil tissue sections in pH8 EDTA for 20 minutes and allow to cool before testing.

IHC staining of FFPE mouse pancreas with Nucleolin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat pancreas with Nucleolin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry testing of human HL60 cells with Nucleolin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Nucleolin antibody.

Immunofluorescent staining of FFPE human U-2 OS cells with Nucleolin antibody (green) and DAPI nuclear stain (blue).

Nucleolin Antibody Rabbit Polyclonal immunofluorescence analysis of human A431 cells. Formalin-fixed cells stained with Nucleolin Antibody Rabbit Polyclonal (green) and Beta Tubulin antibody (red). Green fluorescence highlights nucleolin-positive nuclei and nucleoli, consistent with the expected nuclear and nucleolar localization of Nucleolin / NCL, while Beta Tubulin staining outlines the cytoskeletal network within the cytoplasm.