Nucleolin Antibody for FACS / NCL Flow Cytometry Antibody [clone 364-5 + NCL/902] (V2758)

Description

Nucleolin (NCL) is a multifunctional nucleolar phosphoprotein that plays a central role in ribosome biogenesis, ribosomal RNA transcription, and nucleolar organization. The protein is encoded by the NCL gene on chromosome 2q37 and is highly abundant in the nucleolus of proliferating cells where ribosome production is actively occurring. Nucleolin participates in transcription of ribosomal RNA genes, processing of precursor rRNA transcripts, and assembly of ribonucleoprotein complexes required for ribosome formation. The Nucleolin Antibody for FACS is a mouse monoclonal antibody reagent developed for detection of nucleolin expression in cells using flow cytometry, enabling quantitative analysis of NCL levels across cell populations.

Flow cytometry provides a powerful approach for analyzing intracellular protein expression in thousands to millions of individual cells within a sample. When used as an NCL Flow Cytometry Antibody, nucleolin antibodies allow researchers to evaluate nuclear protein expression patterns, identify proliferative cell populations, and measure changes in nucleolin levels during cellular activation or transformation. Cells are typically fixed and permeabilized prior to staining so that antibodies can access nucleolin within the nucleus and nucleolus. Following staining with a fluorophore-conjugated antibody or fluorescent secondary antibody, nucleolin-positive cells can be detected and quantified by fluorescence intensity during flow cytometric analysis.

Nucleolin contains several structural domains that support its diverse biological functions. The N-terminal acidic domain interacts with chromatin and ribosomal RNA transcription complexes. Four central RNA recognition motifs allow nucleolin to bind ribosomal RNA and other RNA molecules, while the glycine- and arginine-rich C-terminal region facilitates interactions with nucleic acids and nucleolar proteins. Through these domains nucleolin coordinates ribosomal RNA synthesis, RNA processing, and ribonucleoprotein assembly. Because nucleolin expression correlates with ribosome production and cellular proliferation, flow cytometric detection of NCL is frequently used in studies examining cell growth, tumor biology, and nucleolar stress responses.

NCL antibody reagents are commonly described in the literature using several established synonyms for the protein. NCL antibody, also known as nucleolin antibody, C23 nucleolin antibody, and nucleolar protein nucleolin antibody, recognizes a major nucleolar phosphoprotein historically described as nucleolin nucleolar phosphoprotein. The designation C23 originates from early nucleolar protein fractionation experiments in which nucleolin was identified as a prominent nucleolar phosphoprotein associated with ribosomal transcription complexes. These alternative names remain widely used in research focused on nucleolar biology and ribosome synthesis.

Nucleolin is dynamically distributed within cells and can shuttle between the nucleolus, nucleoplasm, cytoplasm, and in some cases the cell surface depending on cellular conditions. In proliferating cells nucleolin expression is often elevated to support increased ribosome production and protein synthesis. Elevated levels of nucleolin are frequently observed in tumor cells where the protein contributes to regulation of transcription, RNA stabilization, and chromatin remodeling. Because flow cytometry can measure fluorescence intensity in individual cells, an NCL Flow Cytometry Antibody provides a useful tool for examining nucleolin expression across heterogeneous cell populations.

Antibodies targeting nucleolin are therefore valuable reagents for cellular analysis workflows including intracellular staining and multiparametric flow cytometry experiments. A Nucleolin Antibody for FACS such as clones 364-5 and NCL/902 can support analysis of nucleolin expression in cultured cells, tumor samples, and immune cell populations, enabling researchers to evaluate nuclear protein distribution and proliferation-associated changes using flow cytometric detection.

Application Notes

Optimal dilution of the Nucleolin Antibody for FACS should be determined by the researcher.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

SUDHL1 cell nuclear lysate (364-5) and recombinant human protein (NCL/902) were used as the immunogen for the Nucleolin antibody cocktail.

Storage

Store the Nucleolin antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

NCL antibody, nucleolin antibody, C23 nucleolin antibody, nucleolar protein nucleolin antibody, nucleolin nucleolar phosphoprotein antibody