Nuclear Antigen Antibody / Pan-Nuclear Marker Antibody [clone NM106] (V3107)

Description

Nuclear Antigen Antibody / Pan-Nuclear Marker Antibody recognizes a broadly expressed nuclear-associated antigen present within the nuclei of cells across multiple tissue types. Unlike antibodies directed against a specific transcription factor, histone, or nuclear enzyme, this antibody functions as a general nuclear marker, enabling visualization of cellular nuclei in normal and malignant tissues. The target antigen is associated with the nuclear compartment and produces characteristic nuclear staining patterns that facilitate identification of cell nuclei within complex tissue environments. As a result, this antibody serves as a useful tool for histology, immunohistochemistry, immunofluorescence, and general cell biology applications requiring reliable nuclear visualization.

The cell nucleus serves as the central repository for genomic DNA and contains numerous proteins involved in gene expression, DNA replication, chromatin organization, and cellular regulation. Accurate identification of nuclei is essential for assessment of tissue architecture, cellular distribution, and subcellular localization studies. Pan-nuclear markers provide investigators with the ability to distinguish nuclear structures from cytoplasmic and membrane-associated compartments, making them valuable components of multiplex staining panels and tissue characterization workflows.

Nuclear Antigen Antibody is particularly useful when evaluating tissue morphology and cellular organization. Because the recognized antigen is broadly expressed, the antibody can be used to visualize nuclei in a wide variety of cell types without requiring cell-type specific markers. This broad reactivity makes it suitable for identification of nuclear compartments in both normal tissues and pathological specimens. The antibody may also be used to assist in interpretation of subcellular localization studies by providing a clear nuclear reference signal for comparison with other targets.

In addition to tissue staining applications, pan-nuclear markers are valuable tools for studies involving cultured cells, frozen sections, paraformaldehyde-fixed preparations, and subcellular fractionation experiments. Reliable nuclear detection allows investigators to evaluate nuclear integrity, monitor cellular distribution, and assess localization of proteins relative to the nuclear compartment. These applications have established nuclear marker antibodies as important reagents in cell biology, pathology, and biomedical research laboratories.

At NSJ Bioreagents, we provide highly validated antibodies for cell biology, pathology, and research applications. Nuclear Antigen Antibody / Pan-Nuclear Marker Antibody is useful for nuclear visualization, tissue architecture assessment, subcellular localization studies, and identification of nuclei in normal and malignant tissues. Continued use of pan-nuclear markers helps researchers better understand cellular organization and tissue structure in a wide variety of experimental systems.

Nuclear Antigen Antibody functions as a broad pan-nuclear marker for identifying cell nuclei and assessing tissue architecture, making it a relevant target within our Nuclear Marker Antibody collection.

Application Notes

Optimal dilution of the Nuclear Antigen Antibody / Pan-Nuclear Marker Antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Nuclei of HL60 cells were used as the immunogen for the Nuclear Marker antibody.

Storage

Store the Nuclear Marker antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

Pan-Nuclear Marker Antibody, Nuclear Marker Antibody, Nuclear Protein Marker Antibody, Cell Nucleus Marker Antibody, Universal Nuclear Marker Antibody, Nuclear Compartment Marker Antibody