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Home >> Antibodies >> NUBP1 Antibody / Nucleotide-binding protein 1

NUBP1 Antibody / Nucleotide-binding protein 1 (RQ8380)

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  Catalog No Formulation Size Price (USD)  
Image RQ8380 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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Immunofluorescent staining of FFPE human HeLa cells with NUBP1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human HeLa cells with NUBP1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) HeLa, 2) A549, 3) RT4, 4) U-251, 5) COLO-320, 6) HepG2, 7) U-2 OS and 8) 293T cell lysate with NUBP1 antibody. Predicted molecular weight ~35 kDa.
Flow cytometry testing of fixed and permeabilized human K562 cells with NUBP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NUBP1 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P53384
Localization Cytoplasm, nuclear
Applications Western Blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Flow Cytometry : 1-3ug/million cells
ELISA : 0.1-0.5ug/ml
Limitations This NUBP1 antibody is available for research use only.
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Description

NUBP1 also known as NBP as is expressed in several tissues with the highest being in the lung and testis. NUBP1 appears to interact with actin and the cytoskeleton and is required for centrosome duplication but it is also need for proper synthesis of iron sulfur proteins and the protein also binds ATP. NUBP1 has roles in cell growth, cellular iron homeostasis, centrosome localization, iron-sulfur cluster assembly, and apolipoprotein modification. NUBP1 is localized to the cytoplasm. Interestingly too, recent research indicates that mutations in NUBP1 in mice lead defects in lung development and eye cataracts, many of which are traced back to NUBP1 role in regulating centrosome dynamics and microtubule organization.

Application Notes

Optimal dilution of the NUBP1 antibody should be determined by the researcher.

Immunogen

An E.coli-derived human recombinant protein (M1-Q311) was used as the immunogen for the NUBP1 antibody.

Storage

After reconstitution, the NUBP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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