NTRK2 Antibody / Neurotrophic tyrosine kinase receptor type 2 / TrkB [clone NTRK2/4672] (V5271)

Immunohistochemistry analysis of NTRK2 antibody (clone NTRK2/4672) in FFPE human colon tissue. HRP-DAB brown chromogenic staining is observed predominantly in the epithelial cells lining the colonic glands, with cytoplasmic and membranous localization in glandular epithelial cells. Surrounding stromal cells show minimal staining. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min followed by cooling prior to immunostaining.

SDS-PAGE analysis of purified, BSA-free NTRK2 antibody (clone NTRK2/4672) as confirmation of integrity and purity.

Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using NTRK2 antibody (clone NTRK2/4672). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.