NSMCE2 Antibody / NSE2 SUMO Ligase Component (RQ8343)

NSMCE2 Antibody IF. Immunofluorescence analysis of NSE2 / NSMCE2 expression in human U-2 OS cells using NSMCE2 Antibody. FFPE cells show green fluorescence localized predominantly to the nucleus, consistent with the role of NSMCE2 as a chromatin-associated SUMO ligase component involved in DNA repair and genome stability. Beta Tubulin antibody (red) highlights the cytoskeletal microtubule network, and nuclei are visualized by DAPI (blue). Antigen retrieval was performed by steaming sections in pH 6 citrate buffer for 20 minutes.

Western blot testing of human 1) MCF7, 2) U-2 OS, 2) A431, 4) K562, 5) A549 and 6) SiHa cell lysate with NSMCE2 antibody. Predicted molecular weight ~28 kDa.

NSMCE2 Antibody Human Sample WB. Western blot analysis of NSE2 / NSMCE2 expression using NSMCE2 Antibody in human cell lysates. Lane 1: MCF7, Lane 2: HEL, Lane 3: U-2 OS, Lane 4: PC-3. A band is detected at approximately 28 kDa, consistent with the predicted molecular weight of NSMCE2 / NSE2, a SUMO ligase component of the SMC5-SMC6 complex involved in DNA repair and genome stability.

NSMCE2 Antibody for FACS. Flow cytometry analysis of NSE2 / NSMCE2 expression in fixed and permeabilized human PC-3 cells using NSMCE2 antibody at 1 ug per million cells. The blue histogram shows a rightward shift compared to the isotype control (green) and unstained cells (red), indicating positive intracellular detection of NSMCE2. Cells were blocked with goat sera prior to antibody incubation to reduce non-specific binding.

NSMCE2 Antibody for IP. Immunoprecipitation of NSE2 / NSMCE2 from human MCF7 whole cell lysate using NSMCE2 antibody. Lane 1: input lysate (10%), Lane 2: control IgG immunoprecipitation, Lane 3: NSMCE2 antibody immunoprecipitation. A band corresponding to NSMCE2 is detected at approximately 28 kDa in the specific immunoprecipitation lane, while the IgG heavy chain is visible at ~50 kDa. These results demonstrate successful enrichment of NSMCE2 using the NSMCE2 antibody.