NSF Antibody / Vesicle-Fusing ATPase Antibody (RQ4040)

NSF Antibody Rat Brain and Mouse Tissue WB. Western blot analysis of NSF Antibody / Vesicle-Fusing ATPase Antibody detected a specific band at approximately 83 kDa, consistent with the expected molecular weight of NSF. Lanes: (1) rat brain, (2) rat testis, (3) mouse brain, (4) mouse testis, (5) mouse spleen, and (6) mouse lung lysates. Strong immunoreactive bands are observed across rat and mouse tissues, demonstrating cross-species recognition of endogenous NSF, with highest expression evident in brain tissue, consistent with the essential role of NSF in synaptic vesicle recycling and intracellular membrane trafficking. This NSF Antibody supports western blot studies of vesicle-fusing ATPase expression, SNARE complex recycling, membrane fusion, and neurobiology.

NSF Antibody Human Tissue and Cancer Cell WB. Western blot analysis of NSF Antibody / Vesicle-Fusing ATPase Antibody detected a prominent band at approximately 83 kDa, consistent with the expected molecular weight of NSF. Lanes: (1) human placenta, (2) human K562, (3) human A549, (4) human MCF-7, (5) human U2OS, (6) human SW620, (7) human 22Rv1, and (8) human HepG2 lysates. Robust NSF expression is detected across all human tissue and cancer cell samples, consistent with the ubiquitous requirement for NSF in ATP-dependent SNARE complex disassembly and intracellular membrane trafficking. This NSF Antibody supports western blot studies of vesicle-fusing ATPase expression, SNARE complex recycling, membrane fusion, and intracellular transport.

NSF Antibody Human Colon Cancer IHC. FFPE human colon cancer tissue was stained with NSF Antibody / Vesicle-Fusing ATPase Antibody following heat-induced epitope retrieval in pH8 EDTA buffer. The image demonstrates strong predominantly cytoplasmic staining throughout malignant epithelial cells with minimal background in the surrounding stromal tissue, consistent with the ubiquitous role of NSF in ATP-dependent membrane trafficking and SNARE complex recycling. This NSF Antibody supports immunohistochemical studies of vesicle-fusing ATPase expression, intracellular transport, membrane fusion, and cancer biology.

NSF Antibody Mouse Brain IHC. FFPE mouse brain tissue was stained with NSF Antibody / Vesicle-Fusing ATPase Antibody following heat-induced epitope retrieval in pH8 EDTA buffer. The image demonstrates widespread moderate to strong cytoplasmic staining throughout neuronal cell bodies and neuropil with minimal background, consistent with the abundant expression of NSF in the central nervous system where it supports ATP-dependent SNARE complex recycling and synaptic vesicle trafficking. This NSF Antibody supports immunohistochemical studies of vesicle-fusing ATPase expression, intracellular membrane trafficking, synaptic vesicle recycling, and neurobiology.

NSF Antibody Rat Brain IHC. FFPE rat brain tissue was stained with NSF Antibody / Vesicle-Fusing ATPase Antibody following heat-induced epitope retrieval in pH8 EDTA buffer. The image demonstrates widespread moderate to strong cytoplasmic staining throughout the brain, including prominent immunoreactivity in neuronal cell layers and surrounding neuropil, with minimal background staining. This distribution is consistent with the high abundance of NSF in neurons, where it functions in ATP-dependent SNARE complex recycling and synaptic vesicle trafficking. This NSF Antibody supports immunohistochemical studies of vesicle-fusing ATPase expression, intracellular membrane trafficking, synaptic vesicle recycling, and neurobiology.

NSF Antibody T47D Cell IF. Immunofluorescent staining of T47D cells was performed with NSF Antibody / Vesicle-Fusing ATPase Antibody following enzyme-mediated antigen retrieval and DAPI nuclear counterstaining. The image demonstrates diffuse cytoplasmic staining with relatively little nuclear signal, consistent with the intracellular localization of NSF in membrane trafficking pathways. This NSF Antibody supports immunofluorescence studies of vesicle-fusing ATPase expression, SNARE complex recycling, intracellular membrane trafficking, and vesicle fusion.