- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
NSE Antibody [clone NSEL1-1] (V7210)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V7210-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 559 | |
|
|
V7210-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 259 | |
|
|
V7210SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2b |
| Clone Name | NSEL1-1 |
| Purity | Protein G affinity chromatography |
| Buffer | 1X PBS, pH 7.4 |
| UniProt | P09104 |
| Gene ID | 2026 |
| Localization | Cytoplasmic |
| Applications | Western Blot : 1-2ug/ml Immunohistochemistry (FFPE) : 0.5-1ug/ml |
| Limitations | This NSE antibody is available for research use only. |
|
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NSE Antibody recognizes a protein of about 50kDa, which is identified as gamma-enolase/neuron specific enolase/enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
For detection of neuron-specific enolase (NSE), also known as gamma enolase, across tissue types, see our NSE antibody.
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NSE antibody to be titered up or down for optimal performance.
Amino acids 416-433 of human Neuron-specific Enolase were used as the immunogen for this NSE antibody.
Store the NSE antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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