NSE Antibody for IF / ENO2 Immunofluorescence Antibody (RQ4566)

NSE Antibody for IF human A431 cell immunofluorescence staining. Immunofluorescence analysis of ENO2 expression in FFPE human A431 cells using NSE Antibody for IF demonstrates cytoplasmic green fluorescence consistent with Neuron Specific Enolase localization, while nuclei are counterstained with DAPI (blue). The staining pattern highlights ENO2-positive cells with clear cytoplasmic distribution and minimal background. Heat-induced epitope retrieval was performed using pH 6 citrate buffer with steam treatment for 20 min prior to staining.

NSE Antibody Human Pancreatic Cancer IHC. Immunohistochemistry staining of FFPE human pancreatic cancer with NSE antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

NSE Antibody Human Lung Cancer IHC. Immunohistochemistry staining of FFPE human lung cancer with NSE antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

IHC staining of FFPE human placenta with NSE antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

NSE Antibody Mouse Brain IHC. Immunohistochemistry staining of FFPE mouse brain with NSE antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

NSE Antibody Rat Brain IHC. Immunohistochemistry staining of FFPE rat brain with NSE antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human 1) 22RV1, 2) U-2 OS, 3) A431, 4) HepG2, 5) A549, 6) SHG-44, 7) rat brain and 8) mouse brain lysate with NSE antibody at 0.5ug/ml. Predicted molecular weight ~47 kDa.

NSE Antibody FACS. Flow cytometry testing of human A431 cells with NSE antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NSE antibody.