- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
NSE Antibody / Microarray Specificity Validated Antibody [clone ENO2/6680] (V4211)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V4211-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
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V4211-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
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V4211SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2, kappa |
| Clone Name | ENO2/6680 |
| Purity | Protein A/G affinity |
| UniProt | P09104 |
| Localization | Cytoplasm |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT |
| Limitations | This NSE Antibody / Microarray Specificity Validated Antibody is available for research use only. |
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Neuron-specific enolase (NSE), also known as Gamma-enolase or ENO2, is a glycolytic enzyme predominantly expressed in neurons and neuroendocrine cells, where it serves as a widely used marker of neuronal differentiation and neuroendocrine lineage. NSE is localized to the cytoplasm and is frequently used to identify neuronal and neuroendocrine cell populations in both normal and disease contexts. Accurate detection of ENO2 is particularly important due to the presence of closely related enolase isoforms, including alpha-enolase (ENO1) and beta-enolase (ENO3), which share significant sequence homology but exhibit distinct tissue-specific expression patterns.
NSE antibody, also referred to as ENO2 antibody or Gamma-enolase antibody in the literature, recognizes a cytoplasmic protein enriched in neuronal and neuroendocrine tissues. The NSE Antibody clone ENO2/6680 is differentiated by microarray-based specificity validation, providing a high-confidence assessment of selective ENO2 recognition. Protein microarray analysis demonstrates strong and preferential binding to ENO2 relative to other proteins, including structurally related enolase family members, supporting minimal cross-reactivity and improved target specificity.
This level of specificity is critical in applications where accurate protein identification is required, particularly in complex biological samples containing multiple enolase isoforms or heterogeneous cell populations. By reducing off-target binding, this antibody supports more reliable interpretation of staining patterns and signal detection, enabling clearer distinction between neuronal, neuroendocrine, and non-neuronal cell types. This is especially important in studies of tumor tissues, where overlapping marker expression and cellular heterogeneity can complicate data analysis.
Microarray-based validation provides a comprehensive and unbiased approach to evaluating antibody specificity across a large panel of proteins under standardized conditions. The strong signal intensity and top-ranking binding to ENO2 observed for clone ENO2/6680 reinforce its selective recognition profile and suitability for applications requiring validated antibody performance. This approach complements traditional validation methods by offering a broader view of potential cross-reactivity and binding behavior.
Clone ENO2/6680 enables consistent and reproducible detection of Neuron Specific Enolase in research applications where specificity is a primary concern. Its validated binding profile supports confident use in studies of neuronal biology, neuroendocrine differentiation, and protein expression analysis, particularly in settings where distinguishing closely related proteins is essential.
This antibody targets Neuron Specific Enolase in research applications requiring high-confidence, specificity-validated detection of neuronal and neuroendocrine markers, making it well suited for studies of protein expression, cellular identity, and lineage-specific analysis.
This antibody is part of the Gamma-enolase antibody collection, where additional ENO2 antibodies for immunohistochemistry can be explored.
Optimal dilution of the NSE Antibody / Microarray Specificity Validated Antibody should be determined by the researcher.
A recombinant fragment of human protein (within amino acids 416-433) was used as the immunogen for the NSE antibody.
Aliquot the NSE antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
NSE antibody, Neuron Specific Enolase antibody, ENO2 antibody, Gamma-enolase antibody, specificity validated NSE antibody, ENO2 specificity antibody
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