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Home >> Antibodies >> NRAS Antibody

NRAS Antibody (F50733)

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  Catalog No Formulation Size Price (USD)  
Image F50733-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F50733-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot analysis of NRAS antibody and CEM lysate. Predicted molecular weight ~21 kDa.
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Western blot analysis of NRAS antibody and CEM lysate. Predicted molecular weight ~21 kDa.
Western blot analysis of NRAS antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected with the NRAS gene (2).
NRAS antibody flow cytometric analysis of NCI-H460 cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Confocal immunofluorescent analysis of NRAS antibody with NCI-H460 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Mouse, Rat, Pig, Chicken
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P01111
Applications Western blot : 1:1000
Flow Cytometry : 1:10-1:50
Immunofluorescence : 1:10-1:50
Limitations This NRAS antibody is available for research use only.
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Description

NRAS is a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. This protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in the gene encoding this protein are a cause of juvenile myelomonocytic leukemia (JMML).

Application Notes

Titration of the NRAS antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 72-101 from the human protein was used as the immunogen for this NRAS antibody.

Storage

Aliquot the NRAS antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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