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Home >> Antibodies >> NPPA Antibody / Atrial Natriuretic Peptide/Factor

NPPA Antibody / Atrial Natriuretic Peptide/Factor (F54517)

  Catalog No Formulation Size Price (USD)  
Image F54517-0.2ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.2 ml 399
Image
F54517-0.05ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.05 ml 185
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IHC testing of FFPE human prostate carcinoma tissue with NPPA antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of fixed and permeabilized human RD cells with NPPA antibody; Blue=isotype control, Green = NPPA antibody.
Western blot testing of 1) human fetal heart and 2) mouse heart lysate with NPPA antibody. Predicted molecular weight ~17 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt P01160
Applications Western blot : 1:500-1:2000
Immunohistochemistry (FFPE) : 1:25
Flow cytometry : 1:25 (1x10e6 cells)
Limitations This NPPA antibody is available for research use only.
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Description

NPPA belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor(containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity.

Application Notes

The stated application concentrations are suggested starting points. Titration of the NPPA antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 30-56 from the human protein was used as the immunogen for the NPPA antibody.

Storage

Aliquot the NPPA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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