NOX5 Antibody / Reactive Oxygen Species Enzyme Antibody (RQ5745)

NOX5 Antibody Human SiHa Cell IF. Immunofluorescent staining of FFPE human SiHa cells with NOX5 antibody (green). Heat-induced epitope retrieval (HIER) was performed by steaming sections in pH 6 citrate buffer for 20 minutes prior to staining. Nuclei are counterstained with DAPI (blue). NOX5 immunoreactivity is observed predominantly within the cytoplasm with diffuse intracellular staining, consistent with the expected localization of this reactive oxygen species enzyme involved in calcium-dependent redox signaling.

NOX5 Antibody Human Lung Cancer IHC. Immunohistochemical staining of FFPE human lung cancer tissue with NOX5 antibody. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes and allowing them to cool prior to staining. Strong NOX5 immunoreactivity is observed predominantly within the cytoplasm and cell membranes of malignant cells, consistent with the expected localization of this reactive oxygen species enzyme involved in calcium-dependent redox signaling. Cell nuclei are counterstained with hematoxylin (blue).

NOX5 Antibody Human Cell Line WB. Western blot testing of human 1) Jurkat, 2) Raji, 3) HeLa and 4) 293T cell lysate with NOX5 antibody. Predicted molecular weight: ~86 kDa.

NOX5 Antibody HeLa Cell FACS. Flow cytometry testing of fixed and permeabilized human HeLa cells with NOX5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NOX5 antibody.