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Home >> Antibodies >> NOX5 Antibody

NOX5 Antibody (R31049)

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  Catalog No Formulation Size Price (USD)  
Image R31049 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of NOX5 antibody and Lane 1: 22RV1; 2: PANC; 3: HeLa; 4: SKOV. Expected/observed molecular weight: ~86 kDa.
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Western blot testing of NOX5 antibody and Lane 1: 22RV1; 2: PANC; 3: HeLa; 4: SKOV. Expected/observed molecular weight: ~86 kDa.
IHC-P: NOX5 antibody testing of human breast cancer tissue
ICC testing of NOX5 antibody and HeLa cells
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt Q96PH1
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
Immunocytochemistry : 0.5-1ug/ml
Limitations This NOX5 antibody is available for research use only.
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Description

NADPH Oxidase 5, also known as NOX5A or NOX5B, is a protein which in humans is encoded by the NOX5 gene. It is a NADPH oxidase that generates superoxide and functions as an H+ channel in a Ca2+-dependent manner. Banfi et al.(2001) found that, when heterologously expressed, NOX5 was quiescent in unstimulated cells. However, in response to elevations of the cytosolic Ca2+ concentration, it generated large amounts of superoxide. Using RT-PCR and Southern and Western blot analyses, Kamiguti et al.(2005) identified NOX5 as a flavin-containing Ca2+-dependent oxidase present in hairy leukemic cells(HC), but not normal B cells.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the NOX5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the C-terminus of human NADPH Oxidase 5 (KVLKGHCEKFGFRFFQENF) was used as the immunogen for this NOX5 antibody.

Storage

After reconstitution, the NOX5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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