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Home >> Antibodies >> NOX2 Antibody/ gp91phox / CYBB

NOX2 Antibody/ gp91phox / CYBB (R30752)

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  Catalog No Formulation Size Price (USD)  
Image R30752 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE mouse spleen tissue with NOX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE mouse spleen tissue with NOX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat spleen tissue with NOX2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human U937 cell lysate with NOX2 antibody. Predicted molecular weight ~65 kDa, can be observed at ~85 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P04839
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Limitations This NOX2 antibody is available for research use only.
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Description

NADPH Oxidase 2, also called CYBB, p91-PHOX or GP91-1, is an essential component of phagocytic NADPH-oxidase, a membrane-bound enzyme complex that generates large quantities of microbicidal superoxide and other oxidants upon activation. NOX2 assembled on DC phagosomes in a gp91-phox subunit-dependent manner, and reactive oxygen species were produced in a more sustained manner in immature DC phagosomes than in macrophage phagosomes. As a major player in innate immune responses in neutrophils, it is also involved in adaptive immunity through its activity in DCs. In heart cells, physiologic stretch rapidly activates reduced-form NOX2 to produce reactive oxygen species (ROS) in a process dependent on microtubules (X-ROS signaling).

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the NOX2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the middle region of human NOX2 (YWLCRDTHAFEWFADLLQ) was used as the immunogen for this NOX2 antibody (100% homologous in human, mouse and rat).

Storage

After reconstitution, the NOX2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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