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Home >> Antibodies >> NOVA2 Antibody

NOVA2 Antibody (RQ5998)

  Catalog No Formulation Size Price (USD)  
Image RQ5998 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human appendicitis tissue with NOVA2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain with NOVA2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain with NOVA2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain with NOVA2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) U-2 OS, 2) HL60, 3) ThP-1, 4) rat brain, 5) rat spleen, 6) rat lung and 7) mouse brain lysate with NOVA2 antibody. Expected molecular weight: 70-80 kDa with a 50-55 kDa band also sometimes seen (Ref 1).
Flow cytometry testing of human A549 cells with NOVA2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NOVA2 antibody.
Flow cytometry testing of mouse HEPA1-6 cells with NOVA2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NOVA2 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q9UNW9
Localization Nuclear
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry : 1-2ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This NOVA2 antibody is available for research use only.
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Description

NOVA alternative splicing regulator 2 is a protein that in humans is encoded by the NOVA2 gene. It is mapped to 19q13.32. NOVA2 may regulate RNA splicing or metabolism in a specific subset of developing neurons. It binds single strand RNA.

Application Notes

Optimal dilution of the NOVA2 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids M1-Q205) was used as the immunogen for the NOVA2 antibody.

Storage

After reconstitution, the NOVA2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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