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Home >> Antibodies >> NNT Antibody

NNT Antibody (RQ5901)

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  Catalog No Formulation Size Price (USD)  
Image RQ5901 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human U-2 OS cells with NNT antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human U-2 OS cells with NNT antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) rat liver, 2) rat kidney, 3) rat heart, 4) mouse liver, 5) mouse kidney, 6) mouse heart, 7) human HeLa, 8) monkey liver and 9) monkey kidney lysate with NNT antibody. Predicted molecular weight ~114 kDa.
Flow cytometry testing of human ThP-1 cells with NNT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NNT antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat, Monkey
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q13423
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This NNT antibody is available for research use only.
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Description

This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification.

Application Notes

Optimal dilution of the NNT antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids C44-A350) was used as the immunogen for the NNT antibody.

Storage

After reconstitution, the NNT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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