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Home >> Antibodies >> NME7 Antibody / Nucleoside diphosphate kinase 7

NME7 Antibody / Nucleoside diphosphate kinase 7 (F54961)

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  Catalog No Formulation Size Price (USD)  
Image F54961-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54961-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of human T-47D cell lysate with NME7 antibody. Predicted molecular weight ~43 kDa.
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Western blot testing of human T-47D cell lysate with NME7 antibody. Predicted molecular weight ~43 kDa.
Western blot testing of human A549 cell lysate with NME7 antibody. Predicted molecular weight ~43 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with NME7 antibody.
IHC testing of FFPE human cancer tissue with NME7 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt Q9Y5B8
Localization Cytoplasmic
Applications Western blot : 1:500-1:1000
Immunohistochemistry (FFPE) : 1:50-1:100
Limitations This NME7 antibody is available for research use only.
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Description

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

Application Notes

The stated application concentrations are suggested starting points. Titration of the NME7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 25-55 from the human protein was used as the immunogen for the NME7 antibody.

Storage

Aliquot the NME7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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