NME2 Antibody / Nucleoside diphosphate kinase B / NKDB [clone NME2/6433] (V4517)

IHC analysis of NME2 / Nucleoside diphosphate kinase B antibody in human skin tissue. Formalin-fixed, paraffin-embedded human skin shows cytoplasmic HRP-DAB brown chromogenic staining predominantly within epidermal keratinocytes, with weaker staining in the dermal compartment. Clone NME2/6433 demonstrates specific signal following heat-induced epitope retrieval in pH 9 10mM Tris with 1mM EDTA for 20 min. The inset negative control using PBS instead of primary antibody shows no specific staining.

Western blot testing of human 293T and MCF-7 cell lysate with NME2 antibody (clone NME2/6433). Predicted molecular weight ~17 kDa.

Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using NME2 antibody (clone NME2/6433). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.